The determination of licarbazepine concentration in patients' plasma by RP-HPLC
ZHANG Guo-yang
HE Yin-zhou
WANG Li
Abstract：Objective: To set up the HPLC method for determination of licarbazepine concentration in patient's plasma. Methods: The residues were analyzed by RP-HPLC method. Waters sunfire C18 column (4.6 mm × 250 mm, 5 μm) was used, methanol-water-trifluoroacetic acid (600 : 400 : 0.5) was used as mobile phase. The flow rate was 1.0 mL·min-1. The detection wavelength was set at 230 nm and the column temperature was 40 ℃ . The sample size was 20 μL. Cyproheptadine hydrochloride was used as internal standard. Results: Linear regression analysis was made by licarbazepine serum concentration (C) and the peak area ratio (y) of licarbazepine vs internal standard. The regression equation was C = 1.227 × 102 y - 0.364 (r2 = 0.9996, n = 7). The results showed that the recovery ratio of licarbazepine was high in the range of 5.0 - 100.0 μg·mL-1 (average recovery rate = 99.80%, RSD = 1.25%). Conclusion: The method provided a simple and precise way for monitoring plasma concentration level of licarbazepine, and this method can be used for therapeutic drug monitoring of licarbazepine.
Keywords：LicarbazepineRP-HPLCBlood drug concentrationTherapeutic drug monitoring
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 267-270 )