Simultaneous determination of six main constituents in Jinsangsanjie capsules by HPLC gradient elution
TAN Xiong-si
PANG Wu-yao
Abstract：Objective: To establish the quantitative method to determine six main constituents simultaneously in Jinsangsanjie capsules by HPLC gradient elution. Methods: The separation was performed on Venusil MP C18 chromatographic column by HPLC gradient elution. The mobile phase consisted of acetonitrile (A) and 0.03% phosphoric acid solution (B). Gradient elution was as follows: 0 - 9 min 15.0%A, 9 - 16 min 15.0%→21.0%A, 16 - 22 min 21.0%→36.0%A, 22 - 30 min 36.0%→55.0%A, 30 - 37 min 55.0%→75.0%A, 37 - 45 min 75.0%→15.0%A. The flow rate was 0.8 mL·min-1. The column temperature was set at 30 ℃ . The sample quantity was 10 μL. The detection wavelength were set at 210 nm (amygdalin, harpagide), 280 nm (angoroside C, harpagoside) and 208 nm (24-acetate alisol A, 23-acetate alisol B). Results: Amygdalin, harpagide, angoroside C, harpagoside, 24-acetate alisol A and 23-acetate alisol B had a good linear relationship in a certain linear range (r ≥ 0.9992) respectively. The average recovery was 96.77% - 100.05% and the corresponding RSD was 0.60% - 1.55%. The precision and the repeatability were good. Test solution was stable at room temperature within 12 h. Conclusion: The method was reliable, simple. It could be used for the simultaneous determination of 6 main components in Jinsangsanjie capsules.
Keywords：Jinsangsanjie capsulesAmygdalinHarpagideAngoroside CHarpagoside24-acetate alisol A23-acetate alisol B
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 263-266 )