Determination of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside inXinshenan granules by HPLC
FAN Jin-zhao
DING Xiao-fang
BAI Lin
LI Xin
Abstract：Objective: To establish a method for determination of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside in Xinshenan granules. Methods: Diamonsil C18 column (250mm × 4.6mm, 5 μm) was used with a mobile phase consisting of acetonitrile-water (16 : 84). The detection wavelength was 320 nm with a lfow rate of 1.0 mL·min-1. Column temperature was 35℃. Results:The 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside had a good linear relationship within the range of 3.7674 – 25.1160 μg·mL-1 (r = 1). The average recovery was 99.80%, RSD was 1.47% (n = 9).Conclusion:This method is simple, accurate and high sensitive, which can be used for content determination of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside inXinshenan granules.
Keywords：HPLCXinshenan granules2354′-tetrahydroxystilbene-2-O-β-D- glucosideContent determination
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 209-211 )