Study on the inhibitory effect of extracts from Patrinia heterophylla Bunge on leukemic cells in vitro
LIU Li
MU Li-hua
LIU Ping
Abstract：Objective: To study the inhibitory effect of extract from Patrinia heterophylla Bunge on the proliferation of leukemic cells in vitro. Methods: Isolated compounds were obtained from the whole plant Patrinia heterophylla Bunge through the means of chromatographic separation. The leukemic cells HL-60, K562 and Jurkat were cultured with different concentrations of extract from Patrinia extracts and compounds. CCK-8 assay was used to detect the inhibition of those extracts and compounds on human leukemic cell HL-60, K562 and Jurkat. And the effect of compound 3 on K562 cell cycle and apoptosis were detected by flow cytometry. Results: Both extracts and monomeric compounds had a certain dose-dependent inhibitory effect on the proliferation of leukemic cells. Different concentration of compound 3 could induce different degree of apoptosis of K562 cells. The early apoptosis rate was significantly higher than that of the control group (P < 0.01). The percentage of cells in G0/G1 phase increased from 42.34% to 59.48% and 64.72%, which was significantly different from that of the control group (P < 0.01). Conclusion: The Patrinia heterophylla Bunge has obvious inhibitory effect on the proliferation of leukemic cells in vitro. Compound 3 can induce the apoptosis of K562 cells and block the cell cycle in G0/G1 phase. The basis of antitumor pharmacological effects is likely to be related to the iridoid components.
Keywords：Patrinia heterophylla BungeLeukaemiaCell apoptosisIridoid
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 150-153 )