Determination of notoginsenoside R1 in Baoyuandan by HPLC
REN Wei
LI Xin
BAI Lin
FAN Jin-zhao
HUANG Xiao-wu
Abstract：Objective: To establish the HPLC method for content determination of notoginsenoside R1 in Baoyuandan. Methods: HPLC analysis was carried out on Dikma Diamonsil C18 column (250 mm × 4.6 mm, 5 μm) and with gradient elution of acetonitrile and water. The detection wavelength was 203 nm, the flow rate was 1.0 mL·min-1 and the column temperature was 40℃. Results: Notoginsenoside R1 showed a good linear relationship in the range of 38.0-380.0 μg·mL-1 (r = 0.9999). The recovery and RSD of notoginsenoside R1 in Baoyuandan (n = 9) were 99.7% and 1.04% respectively. Conclusion: The method is simple, accurate and repeatable, which is suitable for the content determination of notoginsenoside R1 in Baoyuandan.
Keywords：HPLCBaoyuandanNotoginsenoside R1Content determination
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 81-83 )