Mechanism research of amifostine on inducing the differentiation of human megakaryoblastic leukemia Dami cell line
CHI Xiao-hua
WANG Hai-ying
YANG Bo
TIAN Huan-huan
CAI Li-li
LIU Cui
LU Xue-chun
TONG Wei-hang
Abstract：Objective:To explore the mechanism of amifostine on inducing the differentiation of human megakaryoblastic leukemia Dami cell line.Methods: The human megakaryoblastic leukemia Dami cell line were exposed to amifostine (1 mmol·L-1) for 12 days. At different time points (0、4 d、8 d、12 d), the expression of megakaryocytic differentiation-associated transcription factors such as GATA-binding factor 1 (GATA-1) and nuclear factor erythroid 2 (NF-E2) were detected by RT-PCR and Western blot. Then in order to measure the distribution of the two transcription factors in nucleoplasm, nuclear and cytoplasmic were isolated, and immunolfuorescence was used.Results:There was no elevation on the mRNA or protein levels of megakaryocytic differentiation-associated transcription factors GATA-1 and NF-E2 by RT-PCR and Western blot, but nuclear import assay revealed an increased nuclear translocation activity of GATA-1 and NF-E2.Conclusion: Amifostine induced the differentiation of Dami cells into mature megakaryocytes via the mechanism involving increased nuclear translocation activity of the transcription factors GATA-1 and NF-E2.
Keywords：AmifostineDami cellsTranscription factorNF-E2GATA-1
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 19-22 )