Determination of quercetin-3-O-sophoroside in rat cerebrospinal fluid by microemulsion liquid chromatography
ZHUANG Lu-jiang
LIU Jin-yan
SUN Jiang-bing
ZHOU Jun
Abstract：Objective:To establish a microemulsion liquid chromatography method for the determination of quercetin-3-O-sophoroside in rat cerebrospinal lfuid, and further to apply this method to pharmacokinetics study of quercetin-3-O-sophoroside in rats.Methods: After intravenous injection of quercetin-3-O-sophoroside, the cerebrospinal fluid concentrations of rats at different time points were determined by microemulsion liquid chromatography. At the same time, the pharmacokinetic parameters were studied.Results:The optimized microemulsion mobile phase consisted of 2.0% (v/v) of Genapol X-080, 2.5% (v/v) n-butyl alcohol, 1.5% (v/v) ethyl acetate, 0.3% (v/v) triethylamine and 93.7% (w/v) of aqueous solution (pH = 5.0). The calibration curve for quercetin-3-O-sophoroside was linear in the range of 0.1 – 10.0 μg·mL-1 (r = 0.998 4). The intra-day and inter-day precision (RSD) were below 4.9% and the limits of detection (LOD) was 0.1 μg·mL-1 (S/N = 10). The average rate of recovery was 96.3% and RSD was 5.1%. The pharmacokinetic course of quercetin-3-O-sophoroside in rats iftted the two-compartment model. The relavent pharmacokinetic parameters were as follows:t1/2α= 7.3 min,t1/2β= 28.7 min, AUC = 804.37 μg·min-1·mL-1,Vd = 0.325 μg·mL-1, MRT =25.8 min.Conclusion: The microemulsion liquid chromatography method was successfully applied to determine the concentration of quercetin-3-O-sophoroside in rat cerebrospinal lfuid and to the pharmacokinetics research in rats.
Keywords：Microemulsion liquid chromatographyQuercetin-3-O-sophorosideCerebrospinal lfuidPharmacokinetics
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 338-341 )