Simultaneous determination of carvacrol, thymol, honokiol and magnolol inXiao'er shuganning syrup by HPLC with gradient elution
SHEN Jian
ZHANG Cheng-xian
Abstract：Objective: To establish the HPLC method for simultaneous determination of carvacrol, thymol, honokiol and magnolol inXiao'er shuganning syrup.Methods: The Eclipse XDB-Cl8 column (250 mm× 4.6 mm, 5 μm) was used, with a mobile phase consisted of methanol (mobile phase A) and 0.05% glacial acetic acid solution (mobile phase B) for gradient elution. The column temperature was 30℃. The lfow rate was 1.0 mL·min-1. The injection volume was 20 μL. Carvacrol and thymol were detected at 274 nm, honokiol and magnolol were detected at 294 nm.Results: Carvacrol, thymol, honokiol and magnolol had good linearities at 6.35–127.00 μg·mL-1 (r = 0.999 3), 4.74– 94.80 μg·mL-1 (r = 0.999 1), 4.35– 87.00 μg·mL-1 (r = 0.999 7 ), 5.19– 103.80 μg·mL-1 (r= 0.999 4), respectively. In precision and reproducibility test, the RSD results of 4 components were less than 2.0%; the average recovery and their RSD results were 98.18% (1.15%), 96.94% (1.44%), 99.03% (0.92%), 97.24% (0.79%), respectively.Conclusion:The HPLC method is simple and reliable with a good repeatability, which can simultaneously determine the contents of carvacrol, thymol, honokiol and magnolol inXiao'er shuganning syrup. This method can be used to evaluate the quality of this preparation.
Keywords：HPLCXiao'er shuganning syrupCarvacrolThymolHonokiolMagnolol
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 217-220 )