Determination of moxifloxacin concentration in pleural effusion of tuberculous pleurisy patients by HPLC
DENG Fei
JIN Jia-gui
WANG Lan-tian
CHEN Zhang
YONG Xiao-lan
Abstract：Objective:To establish a method for the determination of moxilfoxacin concentration in pleural effusion of tuberculous pleurisy patients.Methods: We used high performance liquid chromatography (HPLC) to determine moxifloxacin concentration in pleural effusion of tuberculous pleurisy patients. The determination was performed on Agilent Eclipse XDB-C18 column with mobile phase consisted of acetonitrile-water (0.05% trilfuoroacetic acid, 23︰77) at the lfow rate of 1.0 mL·min-1. UV detection wavelengths were set at 296 nm (0– 2.5 min), 256 nm (2.5 –4.5 min) and 296 nm (4.5–8.0 min), respectively. The column temperature was 25℃ and injection volume was 30 μL.Results: In the range of 0.1 –10.0 μg·mL-1 (r = 0.999 2), moxilfoxacin showed a good linear relation. The lower limit of quantitation was 0.1 μg·mL-1. The inter-day precision RSD of low, medium and high concentration were 2.76%, 2.23% and 2.00%, and intra-day precision RSD were 5.88%, 3.49% and 3.60%, respectively. The extraction recovery of them were 53.00%, 63.26% and 65.42%, and internal standard was 91.51%, respectively.Conclusion: The measuring method adopted in the experiment can accurately determine the concentration of moxifloxacin in pleural effusion of tuberculous pleurisy patients with convenience, high sensitivity and exceptional reproducibility.
Keywords：High performance liquid chromatography (HPLC)MoxifloxacinTuberculous pleurisyPleural effusionPharmacokinetics
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 140-143 )