Determination of three main constituents in Shaozao capsules by RP-HPLC
WANG Yu-ning
MU Li-hua
WANG Shi
WEI Pei-pei
WANG Ru
LIU Ping
Abstract：Objective: To establish the quantitative method of three main constituents in Shaozao capsules by RP-HPLC method. Methods: The separation was performed on Kromasil C18 column (4.6 mm × 250 mm, 5 μm) by RP-HPLC method. The mobile phase consisted of acetonitrile (A) and water (B), elution gradient was as follows: 0 –10 min 10% –19%A; 10 – 16 min 19% – 20%A; 16 – 22 min 20% – 90%A; 22 – 25 min 90%A; 25 – 25.1 min 90% – 10%A; 25.1 –35 min 10%A. The flow rate was 0.8 mL·min-1. The detector was Alltech 2000ES ELSD. The temperature of drift tube was 115 ℃ and the air flow rate was 3.3 L·min-1. Results: The linear ranges of the three components were 0.975 – 5.850 μg (paeoniflorin), 0.060 –0.660 μg (spinosin) and 0.100 –0.600 μg (3,6'-disinapoyl sucrose) respectively. The average recoveries of the above three components in Shaozao capsules were 99.5% (RSD 0.69%), 100.2% (RSD 1.03%), 99.9% (RSD 1.20%) respectively. Conclusion:The method was reliable, simple, and precise, which was suitable for quality control of Shaozao capsules.
Keywords：Shaozao capsulesPaeonilforinSpinosin36'-disinapoyl sucroseRP-HPLC
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 77-79,84 )