Effect of Radix Rehmanniae extract on hyperuricemia mice

LIU Jing; LIU Mei; YANG Bo; MA Jing; LU Yi; QIU Yan

Abstract：Objective:To observe the effect of Radix Rehmanniae extract on the level of serum uric acid in hyperuricemia mice and investigate the mechanism preliminarily.Methods:The intraperitoneal injection of uricase inhibition potassium oxonate was used to induce the hyperuricemia in mice. The mice were randomly divided into model group, Radix Rehmanniae extracts treatment groups (with the doses of 2, 4 and 8 g·kg-1) and the allopurinol positive control group (20 mg·kg-1). The serum levels of uric acid, creatinine, nitric oxide, and the levels of xanthine oxidase (XOD) and adenosine deaminase (ADA) in liver were observed, and the pathological changes in kidney of mice were detected.Results: After 14 days of drug intervention, compared with the model group, the Radix Rehmanniae extract middle and high dose groups reduced the levels of serum uric acid and creatinine (P < 0.01), increased serum levels of nitric oxide (P < 0.05,P < 0.01), and signiifcantly inhibited the liver XOD (P < 0.01). It showed that Radix Rehmanniae extract high dose group signiifcantly inhibited the ADA in hyperuricemia mice (P < 0.05). The results of pathological examination showed that the pathological changes in kidney of all extracts groups were lower than that of the model group. Conclusion: The Radix Rehmanniae extract can reduce the level of serum uric acid in the model of hyperuricemia mice, and the effect is related to the inhibition of the activity of liver XOD and ADA.