Content determination of notoginsenoside R1, ginsenoside Rg1, Re, Rb1 inXuesaitong soft capsules by HPLC
HAN Ya-liang
GU Jin
HE Xin-rong
ZHANG Fei-yan
JI Tie-feng
Abstract：Objective:To establish the method for determining the notoginsenoside R1, ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in Xuesaitong soft capsules by HPLC.Methods: The separation was performed on ZORBAX SB-Aq column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-water with linear gradient elution. The flow rate was 1.0 mL·min-1. The detection wavelength was 203 nm and the column temperature was 25℃.Results: The calibration curves showed good linearity in the range of 0.32–3.20 μg (notoginsenoside R1,r = 1.000 0), 0.72– 7.20 μg (ginsenoside Rg1,r = 1.000 0), 0.21– 2.10 μg (ginsenoside Re,r = 0.999 9), 0.92– 9.20 μg (ginsenoside Rb1,r = 1.000 0). The average recoveries (n = 9) were 100.22%, 100.62%, 100.02%, 100.33% respectively, and RSD were 0.99%, 1.18%, 2.02%, 0.97% respectively.Conclusion: The method is convenient, rapid, economical and accurate, which is suitable for quality control of Xuesaitong soft capsules.
Keywords：Xuesaitong soft capsulesNotoginsenoside R1Ginsenoside Rg1Ginsenoside ReGinsenoside Rb1HPLC
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 268-271 )