Studies on growth inhibition and apoptosis effect of the combined therapy with methionine enkephalin and doxorubicin towards neuroblastoma SH-SY5Y cells
LI Yan-bo
LIU Fei-fei
QU Heng-yan
Abstract：Objective:To observe the combined growth inhibition and apoptosis effect of methionine enkephalin (Met-ENK) and doxorubicin towards neuroblastoma SH-SY5Y cells, in order to provide evidence for the combined therapy with Met-ENK in clinic.Methods: CCK-8 method was adopted to detect the synergistic growth inhibition. Apoptosis was detected by flow cytometry. Based on synergistic growth inhibition, the synergy index (q) was derived by the King formula to define the synergistic effect of Met-ENK and doxorubicin. The SAS9.0 software was adopted to analyze the statistical signiifcance of the data.Results: The growth inhibition ratio of neuroblastoma SH-SY5Y cells in Met-ENK group, doxorubicin group and combined therapy group (Met-ENK and doxorubicin) was 0.29%, 0.71% and 0.82% respectively. The synergistic indexq of Met-ENK and doxorubicin was 2.19 (q > 2.0). Met-ENK showed no effect on apoptosis of SH-SY5Y cells. The apoptosis rate of SH-SY5Y cells was 49.1% in doxorubicin group and significantly increased to 65.2% in combined therapy group (Met-ENK and doxorubicin) with significant difference (P < 0.01). Conclusion: Met-ENK could signiifcantly improve the sensitivity of neuroblastoma SH-SY5Y cells to doxorubicin. Combined therapy with Met-ENK and doxorubicin could significantly inhibit the growth of tumor cells and induce the apoptosis of tumor cells.
Keywords：Methionine enkephalinDoxorubicinCombination therapySynergisticApoptosis
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 265-267 )