Effect of Gandanliangyitang on monoamine neurotransmitter in different brain regions of insomnia model of rats

WANG Yan; ZHANG Gang-qiang; ZHANG Nan; LIU Ping

Abstract：Objective: To study the mechanism of Gandanliangyitang (GDLYT) in the treatment of insomnia and provide reference for clinical use.Methods:A total of 56 SD rats were randomly divided into normal group, model group, diazepam group (0.001 g·kg-1),Bailemian group (0.215 g·kg-1) and GDLYT low dose group (0.925 g·kg-1), middle dose group (1.850 g·kg-1) and high dose group (3.700 g·kg-1). The model of insomnia rats was made successfully by using continuous intraperitoneal injection of parachlorophenylalanine (0.3 g·kg-1). The contents of 5-HT, DA and NE in cortex, hippocampus, corpus striatum, hypothalamus and brainstem of rats were detected by using high performance liquid chromatography with lfuorescence detector.Results: Compared with normal group, in model group, the content of 5-HT in rat cortex and hippocampus, striatum, hypothalamus and brainstem significantly decreased (P < 0.01), DA content in the hypothalamus significantly decreased (P < 0.05), NE content increased in striatum and hypothalamus (P < 0.05). Compared with the model group, in GDLYT treatment group, the content of 5-HT in hippocampus, cortex, striatum and hypothalamus increased signiifcantly, NE content in hypothalamus signiifcantly decreased (P < 0.05). The best dose is 1.850 g·kg-1.Conclusion:GDLYT plays remarkable role on sedative hypnotic, the treatment mechanism of insomnia is probably by increasing the 5-HT content in the brain and decreasing the content of NE in hypothalamus, regulating neuroendocrine system.