Determination of tretinoin and its related substance in tretinoin ointment
CAI Le
YAO Lan
BAI Lin
Abstract：Objective:To establish the HPLC method for determination of tretinoin and its related substance in tretinoin ointment.Methods:HPLC analysis was carried out on a column of Agilent Zorbax SB C18 (250 mm × 4.6 mm, 5 μm) with mobile phase consisted of methanol -2% glacial acetic acid solution (85 : 15), the lfow rate was 1.0 mL·min-1, the detection wavelength was set at 354 nm and column temperature was 45℃.Results:The separation between tretinoin and other impurities was well under this chromatographic condition, and separating degree of tretinoin and isotretinoin was>5. The calibration curve of tretinoin was linear in the ranger of 0.934 4–18.687 2 μg·mL-1 (r = 1.000 0). The average recovery of tretinoin was 99.4% (RSD = 1.44%,n = 9). Conclusion:This method is simple, accurate and feasible, which can be applied as the quantitative method for the determination of tretinoin in tretinoin ointment.
Keywords：HPLCTretinoin ointmentContent determinationTretinoinIsotretinoin
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 144-146 )