Inhibition of compound AG4 fromArdisia gigantifolia Stapf. on xenograft tumor growth of human nasopharyngeal carcinoma in nude mice
CHEN Chao
SUN Yan
DONG Xian-zhe
GUO Dai-hong
ZHENG Xiao-li
MU Li-hua
LIU Ping
Abstract：Objective:To investigate the influence of compound AG4 derived fromArdisia gigantifolia Stapf. on xenograft tumor of human nasopharyngeal carcinoma (NPC) in nude mice.Methods: Human NPC nude mice model were established, and the inhibition rate (IR) was the index to evaluate the tumor growth suppression of AG4in vivo. Simultaneously, general conditions (weight, action, appetite) of nude mice and the expression of Bcl-2, Bad and Bax mRNA by real time PCR method were observed during AG4 interference.Results: The IR of positive control group (cisplatin 2.00 mg·kg-1) and AG4 group (3.02 mg·kg-1) was 49.41% and 42.34% respectively. The effect of AG4 on the weight lose of nude mice was not significant comparing with negative control group, and the toxicity of AG4 to the vital organs (liver, spleen and kidney) and immunity index (spleen index) was not obvious in short period. AG4 could increase the Bax and Bad mRNA expression, and decrease the Bcl-2 mRNA expression.Conclusion:AG4 had obvious inhibition effects on xenograft tumor in human NPC nude mice, and with no outstanding adverse effects, the mechanisms may relate to the induction of tumor apoptosis due to the activation of mitochondria signal pathway.
Keywords：Ardisia gigantifolia Stapf.Nasopharyngeal carcinomaBcl-2 familyNude mice
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 12-15 )