Content determination of diphenhydramine hydrochloride cream by HPLC
BAI Lin
FAN Jin-zhao
CAI Le
XU Feng-hua
Abstract：[ABSTRACT]Objective: To establish the HPLC method for determining the content of diphenhydramine hydrochloride cream.Methods:HPLC analysis was carried out with Platisil C18column (250 mm×4.6 mm, 5μm), the mobile phase consisted of acetonitrile-water-triethylamine (50 : 50 : 0.5), the detection wavelength was 258 nm, the flow rate was 0.8 mL·min-1 and the column temperature was 40℃.Results:The samples were separated well from impurities by this HPLC method. The calibration curve of diphenhydramine hydrochloride was linear in the range of 125.0 – 1 000.0μg·mL-1 (r = 1.000 0). The average recovery and the RSD of diphenhydramine hydrochloride were 98.18%, 0.40% (n = 9), respectively.Conclusion:This method is accurate and feasible, which can be applied as the quantitative method for the determination of diphenhydramine hydrochloride cream.
Keywords：HPLCDiphenhydramine hydrochloride creamContent determination
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 348-350 )