Content determination of salicylic acid in three preparations by HPLC
BAI Lin
CAI Le
XU Feng-hua
WANG Huan-huan
Abstract：Objective:To establish the HPLC method for determining the content of salicylic acid in three preparations (salicylic acid ointment, salicylic acid and sulfur ointment, compound sulfur sublimate ointment). Methods:HPLC analysis was carried out with Diamonsil C18 column (250 mm × 4.6 mm, 5 μm), the mobile phase consisted of methanol-0.1 mol·L-1 sodium dihydrophosphate solution (30 : 70), the detection wavelength was set at 231 nm, the flow rate was 1.0 mL·min-1, the column temperature was 35℃and the sample size was 10μL. Results: The sample was separated well from impurities by this HPLC method. The calibration curve of salicylic acid was linear in the range of 12.32-123.20μg·mL-1 (r=1.000 0). The average recoveries and the RSD of salicylic acid were 100.55%and 0.95%(n=9) in the salicylic acid ointment, 99.96%and 0.96%(n=9) in the salicylic acid and sulfur ointment, 99.13%and 1.15%(n=9) in sulfur sublimate ointment, respectively. Conclusion:This method is accurate and feasible, which can be applied as the quantitative method for the determination of salicylic acid in three preparations.
Keywords：HPLCPreparationSalicylic acidContent determination
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 283-285 )