Determination of tacrolimus in human whole blood by HPLC-MS/MS
ZHAO Guan-ren
SHEN Jian
PENG Ming-li
JIANG Nan
Abstract：Objective:To establish the HPLC-MS/MS method for the determination of tacrolimus in human whole blood. Methods: The diazepam was used as internal standard. Tacrolimus was separated on an Agilent Poroshell 120 SB-C18 column (4.6 mm × 50 mm, 2.7μm) maintained at 40℃with acetonitrile-0.01 mol·L-1 ammonium acetate and 0.5%formic acid as mobile phase by gradient elution. The lfow rate was 0.7 mL·min-1, and the sample size was 5μL. Detection was performed with multiple reaction monitoring (MRM) scan mode measured with positive electrospray ionization (ESI+). The monitoring ions of tacrolimus and diazepam were m/z 821.6→768.5 and m/z 285.1→193.1, respectively. Results:Tacrolimus showed a good linear relationship in the range of 1.49-29.78 ng·mL-1. The accuracies were in the range of 95.19%-99.02%, both the inter-run and intra-run RSD (3 d) were less than 10.00%. Conclusion:The method is simple, rapid and sensitive, which can be used for the therapeutic drug monitoring of tacrolimus.
Keywords：HPLC-MS/MSTacrolimusBlood drug concentration
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 276-278 )