Content determination of camphor in compound camphor ointment by HPLC
BAI Lin
FAN Jin-zhao
XU Feng-hua
WANG Huan-huan
Abstract：Objective: To establish the HPLC method for determining the content of camphor in compound camphor ointment. Methods:The analysis was carried out on a column of Diamonsil C18 (250 mm × 4.6 mm, 5μm) with the mobile phase consisting of methanol-acetonitrile-water (15∶45∶40), the flow rate was 1.0 mL·min-1, the detection wavelength was 289 nm, column temperature was 35℃, the sample injection was 10μL. Results:The samples were separated well from other substances. The calibration curve of camphor was linear in the range of 250.00-2 000.00 μg·mL-1 (r = 1.000 0). The average recovery of camphor in the compound camphor ointment was 99.68%with RSD of 1.21%(n=9). Conclusion: This method is accurate and feasible, which can be applied as the quality control of camphor in the compound camphor ointment.
Keywords：HPLCCompound camphor ointmentCamphorContent determination
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 212-213,258 )