Simultaneous determination of two components in complex ethacridine lactate solution by HPLC
MA Ping
WU Cheng
WANG Jing-cheng
CAO Miao
SUN Ping
ZHANG Li-ping
NIU Hua-ying
Abstract：Objective: To establish the HPLC method for simultaneous determination of ethacridine lactate and benzhydramine hydrochloride in complex ethacridine lactate solution. Methods: The extract was separated by Luna CN column (4.6 mm × 250 mm, 5μm ), and the mobile phase was consisted of acetonitrile-water-triethylamine (50:50:0.5) with the lfow rate of 1.0 mL·min-1. The column temperature was 30℃. The detection wavelength was 230 nm and the injection volume was 20 μL. Results:The calibration curves of ethacridine lactate and benzhydramine hydrochloride were linear in the range of 0.601 2-1.403 0 mg·mL-1 (r = 0.999 4) and 0.148 7 - 0.346 9 mg· mL-1 (r = 0.999 9), respectively. The average recovery of ethacridine lactate was 99.91%with RSD of 1.06%(n=9). The average recovery of benzhydramine hydrochloride was 99.73%with RSD of 1.10%(n=9). Conclusion:The method is simple, accurate and reliable. It can be used as the quality control of complex ethacridine lactate solution.
Keywords：Complex ethacridine lactate solutionEthacridine lactateBenzhydramine hydrochlorideHPLCContent determination
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 149-151 )