Study on the methodology establishment of content determination and stability of representative saponins in Panax notoginseng saponins
LU Yan
PAN Xu
Abstract：Objective:The HPLC method for simultaneous determination of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 in Panax notoginseng saponins (PNS) was established to study the stability of the saponins under different states. Methods:Acetonitrile-water system was used as the mobile phase, and the method speciifcity, linearity, precision, recovery were studied. The stability of the three saponins in different pH solutions and solid state was evaluated. Results: The results of method validation showed good speciifcity, effective separation and quantiifcation, as well as good linearity of the three saponins in their linear range. The precision RSD values were less than 2%, average recovery rates were (98.98 ± 0.60)%, (98.86 ± 0.34)%and (100.19 ± 0.64)%for the three saponins respectively. The stability results showed that the stability of the three saponins was closely related to the pH value under the solution state, with rapid degradation about 80%in 24 h in pH 1.2 solutions and little degradation in the other test medium. In the solid powder state, the stability of saponins was good under the experimental high temperature, high humidity and highlight conditions, while a little moisture absorption was found in the high humidity conditions. Conclusion:The HPLC method was reliable. Stability of three saponins was closely related to the pH value of solution, while they were stable under solid state.
Keywords：Notoginsenoside R1Ginsenoside Rg1Ginsenoside Rb1StabilityGradient elution
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 88-91 )