Antifungal activity of pseudolaric acid B in vitro and its effect on ergosterol biosynthesis in Candida albicans
LIU Li-ying
CHEN Hong
GAO Mo-jie
Abstract：Objective: To study antifungal activity in vitro and the effect of pseudolaric acid B (PLAB) on ergosterol biosynthesis in the cell membranes of Candida albicans. Methods:Microdilution method was used to determine the susceptibility of fungus (Candida albicans, Cryptococcus neoformans, Epidermophyton floccosum) to PLAB. The Candida albicans were incubated for 28 h with different concentrations of PLAB and lfuconazole, and the content of ergosterol was detected by HPLC after saponiifcation and extraction. Results:The MIC50 (minimum inhibition concentrations) of PLAB to Candida albicans, Cryptococcus neoformans, Epidermophyton lfoccosum was 16, 32, 16 μg·mL-1 respectively. PLAB could decrease the content of ergosterol (P<0.05) in Candida albicans with a dose-dependent manner. Conclusion:PLAB had remarkable antifungal activity by inhibiting the ergosterol biosynthesis in fungi.
Keywords：Pseudolaric acid B (PLAB)HPLCCandida albicansErgosterol
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 85-87,88 )