Determination of phenol in menthol-phenol ointment by HPLC
CAI Le
BAI Lin
XU Feng-hua
REN Wei
WANG Huan-huan
Abstract：Objective:To establish the HPLC method for determination of phenol in menthol-phenol ointment. Methods:The phenol in menthol-phenol ointment was extracted by ultrasonic method, and the content of phenol was determined by HPLC method. The analysis was carried out on a column of Diamonsil C18 (250 mm × 4.6 mm, 5μm) with mobile phase consisting of methanol-water (60∶40), the lfow rate was 1.0 mL·min-1. The detection wavelength was set at 271 nm, and the column temperature was 45℃. Results:Phenol showed a good linear relationship in the range of 9-360μg·mL-1 (r=0.999 9). The average recovery was 100.2%with RSD of 0.68%(n=6). Conclusion: The method is simple, accurate and repeatable, which is suitable for the content determination of phenol in menthol-phenol ointment.
Keywords：Menthol-phenol ointmentPhenolHPLCContent determination
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 82-84 )