Preparation of timosaponin B-Ⅱnanofiber membranes and preliminary study on its antitumor activity in vitro

HUO Zhong-hua; YIN Peng; HOU Le-wei; LV Sheng; HU Jun; CHU Zhu-ling; LUAN Rong-gang; HU Guo-qiang

Abstract：Objective: To explore the preparation method of timosaponin B-Ⅱnanofiber membrane, in order to lay a foundation for the subsequent studies in vivo. Methods:Timosaponin B-Ⅱand PLLA at different ratios were made into timosaponin nanoifber membranes;the surface structure and morphology of the drug loaded nanoifber membranes were observed by scanning electron microscopy;the infrared spectra was adopted to determine the compatibility between the drug and the polymer;LC-MS/MS was used to measure timosaponin B-Ⅱlevels in solutions at different time points to draw the drug release curves;CCK-8 assay was used to detect the proliferative activity of tumor cells, in order to evaluate whether the sustained drug release from the drug-loaded nano-iflms could inhibit the proliferation of gastric carcinoma cells. Results: The optimum concentration of timosaponin B-Ⅱfor drug loading was 12%; the electrospinning technique in the preparation of timosaponin B-Ⅱloaded nanofibers could maintain the physical and chemical properties of the both components; timosaponin B-Ⅱhad little effect on the thermal stability of fiber membranes, indicating a good compatibility;the timosaponin B-Ⅱnanoifber membranes could effectively release timosaponin B-Ⅱin the conventional culture solution, the timosaponin B-Ⅱloaded nanoifber membranes can inhibit the proliferation of BGC-823 cells signiifcantly, the differences in the treated groups at 48 hours and 72 hours were signiifcant (P<0.05) compared with the control group. Conclusion:Timosaponin B-Ⅱnanoifber membranes can effectively inhibit the proliferation of BGC-823 cells through the sustained release of the drug.