Content determination of ferulic acid and caffeic acid in Huoxuezhitong solution by HPLC
SHEN Rui
GUO Qi
Abstract：Objective:To establish a method for simultaneous determination for the content of ferulic acid and caffeic acid in Huoxuezhitong solution. Methods:Inertsil ODS-SP C18 column (4.6 mm × 250 mm, 5μm) was used for separation. The mobile phase was consisted of acetonitrile-0.05%phosphoric acid solution (17∶83) at the lfow rate of 1.0 mL·min-1. The column temperature was 30℃. The detection wavelength was 320 nm, and the injection volume was 10μL. Results:The calibration curves of ferulic acid and caffeic acid were linear in the range of 1.766-35.328μg·mL-1 (r=0.999 9) and 0.835-16.704μg·mL-1 (r=0.999 3), respectively. The average recovery of ferulic acid was 100.79%with RSD of 1.73%(n=9). The average recovery of caffeic acid was 101.05%with RSD of 1.87%(n=9). Conclusion:The method is simple, accurate and reproducible, which can be used for the quality control of Huoxuezhitong solution.
Keywords：Huoxuezhitong solutionFerulic acidCaffeic acidHPLCContent determination
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 311-314 )