Effect of methionine enkephalin on growth cycle of neuroblastoma cell and its mechanism research
LI Yan-bo
LIU Fei-fei
LIU Yao
QU Heng-yan
GAO Hong-zhi
LIU Ze-yuan
Abstract：Objective:To study the cell growth inhibition and cell cycle inhibitory mechanisms of methionine enkephalin (Met-ENK) on SH-SY5Y cell. Methods:MTT assay was used for detecting the cell growth inhibition rate, the change of tumor cell cycle was detected by flow cytometry, the levels of intracellular P21 and P53 gene transcript were analyzed by Real-time PCR, the expressions of intracellular P21, P16 and phosphorylated Rb protein were determined using Western blot assay after intervention with Met-ENK. Results:MTT assay showed that Met-ENK could inhibit SH-SY5Y cell growth, and the inhibition rate was 40%at the Met-ENK concentration of 10-5 mol·L-1. The results of flow cytometry confirmed that most of cells were blocked in G0/G1 phase, and the number of cells in G0/G1 phase increased from 63.97%to 87.73%. The transcript levels of P21 gene and P53 gene were three and five times higher than that before intervention with Met-ENK. The expressions of P21 and P16 protein significantly increased, while expression of phosphorylation Rb protein significantly reduced. Conclusion: Met-ENK could inhibit the SH-SY5Y cell proliferation in the G0/G1 phase of the growth cycle by increasing the transcription and protein expression of the cyclin-dependent kinase inhibitor-P21 and tumor suppressor gene-P53 and decreasing the level of Rb protein phosphorylation, which confirmed the mechanism of G0/G1 phase specificity cycle inhibitory effect of Met-ENK.
Keywords：Methionine enkephalinSH-SY5Y cell linesG0/G1 phaseP21P16P53Inhibition
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 261-264 )