The protective effect of dexmedetomidine preconditioning and its effect on complement component 3 in rats with myocardial ischemia reperfusion injury
MO Xiao-fei
CHEN Liu-fang
WAN Jing
WU Yun
KE Jian-juan
Abstract：Objective:To investigate the protective effect and possible mechanism (effect on complement component 3) of dexmedetomidine preconditioning on the myocardium of ischemia and reperfused rats. Methods:Forty-five Sprague-Dawley male rats were divided into sham operation group(Sham group), ischemic-reperfusion group (I/R group) and dexmedetomidine preconditioning group (DEX group) randomly, with 15 rats in each group. Before left anterior descending coronary artery was clamped, DEX group received dexmedetomidine with 5 mL· kg-1 body weight for 2 h (dexmedetomidine was diluted to 1μg· mL-1 with 0.9%sodium chloride injection). While I/R group and Sham group were infused 0.9%sodium chloride injection with 5 mL· kg-1 body weight for 2 h. At the end of 120 min reperfusion, the myocardial tissues were taken for determination of the infarct size and C3 expression using the Western blot method. Results:Compared with the Sham group, the infarct sizes and expressions of C3 increased obviously in the I/R group and DEX group (P< 0.05). Compared with the I/R group, the infarct sizes in DEX group were smaller and expressions of C3 were lower(P<0.05). Conclusion:Dexmedetomidine could attenuate myocardial ischemia-reperfusion injury in rats via suppressing the expression of C3.
Keywords：Ischemic-reperfusionComplement component 3Dexmedetomidine
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 258-260 )