Determination of pinoresinol diglucoside in Jitongning capsules by HPLC
BAI Lin
NIE Shuai
Abstract：Objective:To establish a method for determining the content of pinoresinol diglucoside in Jitongning capsules by HPLC. Methods:Agilent XDB-C18 column (150 mm×4.6 mm, 5μm) was used for separation. The mobile phase consisted of methanol-water (20∶80) at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 227 nm and the column temperature was 40℃. The injection volume was 10μL. Results:The calibration curve of pinoresinol diglucoside was linear in the range of 21.3–213μg·mL-1 (r=0.999 7). The average recovery was 101.2%with the RSD of 0.99%(n=6). Pinoresinol diglucoside was separated well from other interfere substances. The average content of pinoresinol diglucoside in three batches samples was (0.50±0.011 5) mg per capsule. Conclusion:This method is simple, accurate and repeatable, which can be used for quality control of pinoresinol diglucoside in Jitongning capsules.
Keywords：HPLCJitongning capsulesPinoresinol diglucosideContent determination
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 147-149 )