Comparison of two methods on susceptibility testing of tigecycline against Acinetobacter baumannii
NI Wen-tao
LI Yue
LIANG Bei-bei
CAI Yun
CUI Jun-chang
WANG Rui
Abstract：Objective: To study the difference of two testing methods on the susceptibility results of tigecycline against Acinetobacter baumannii. Methods:Minimal inhibitory concentrations (MICs) of 70 isolates of Acinetobacter baumannii were determined by the agar dilution method and broth microdilution method respectively as described by the Clinical and Laboratory Standards Institute (CLSI), and the differences of two methods were compared. Results:The MIC50 and MIC90 values obtained by agar dilution method were 1μg·mL-1, 2μg·mL-1 respectively, and the susceptible, intermediate and resistant rates were 47.1%, 48.6%, 4.3%, respectively; while the MIC50 and MIC90 values obtained by broth microdilution method were 0.25μg·mL-1, 0.5μg·mL-1 respectively, and the susceptible rate was 100%. Weak coherence and correlation were found between the two methods. If broth microdilution method was taken as the reference method, the total error rate of agar dilution method was 52.9%. Conclusion:There was significant difference on susceptible rate between the two methods for the susceptibility testing of tigecycline against Acinetobacter baumannii.
Keywords：Agar dilution methodBroth microdilution methodTigecyclineAcinetobacter baumanniiMinimal inhibitory concentration (MIC)
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 135-137,138 )