Application ofbidirectional labeling method to quantifying carbon utilization in microalgae
LI Haitao
WU Yanyou
ZHAO Lihua
ZHANG Kaiyan
HANG Hongtao
Abstract：August 2015,the Chlorella pyrenoidosawas cultivated in a greenhouse with different inorganic carbons(NaH13CO3 with different δ13C values added) in the culture medium.The δ13C of inorganic carbon in the medium and the biomass of the microalgae were detected on a daily basis.In the meantime,the organic stable carbon isotope compositions of the microalgae was also measured.The proportion of the added inorganic carbon used by microalgae was quantified by comparing their stable carbon isotope compositions using the bidirectional labeling method (NaH13CO3with different δ13 Cvalues was added).This study compared respectively both inorganic carbon and organic carbon of the stable carbon isotope compositions.The results are as follows,it is 0.19 under 5.0 mmol/L NaHCO3,0.37 under 10.0 mmol/L NaHCO3,and 0.57 under 20.0 mmol/L NaHCO3.At last,we analyzed the two methods for quantifying the carbon sources in algae.It is very important to calculate the proportion of different inorganic carbon in the carbon cycle research in karst lakes.
Keywords：inorganic carbon utilizationstable carbon isotopemicroalgae
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 614-618 )