Correlation among miR-499a-5p,Toll-like receptor 2 and major adverse cardiovascular events in patients with coronary heart disease

PAN Linyan; XU Xianjin; WANG Niandong

doi:10.3969/j.issn.1674-4055.2025.11.007

Abstract：Objective To investigate the correlation between expression levels of serum microRNA-499a-5p(miR-499a-5p)and Toll-like receptor 2(TLR2),and major adverse cardiovascular events(MACE)in patients with coronary heart disease(CHD).Methods A total of 130 CHD patients(CHD group)was selected who received treatment in the Department of Cardiovascular Medicine,Anqing Petrochemical Hospital of Nanjing Drum Tower Hospital Group from Aug.2021 to Aug.2022.According to MACE occurrence within 1 y after discharge,they were divided into non-MACE group and t MACE group.In addition,130 healthy people were selected who underwent physical examination in the same hospital during the same period as control group.The severity of coronary artery lesion was evaluated using the Gensini score in CHD patients.The expression levels of serum miR-499a-5p and TLR2 mRNA were detected by qRT-PCR method.The Spearman method was used to analyze the correlation between serum miR-499a-5p and TLR2 mRNA levels,as well as the correlations of each of the two with the Gensini score.Multivariate Logistic regression analysis was applied to identify the influencing factors for MACE in CHD patients.Results Compared with control group,the expression level of miR-499a-5p was significantly decreased in CHD group,while the level of TLR2 was significantly increased(P<0.05).There was a negative correlation between the serum expression levels of miR-499a-5p and TLR2 in CHD patients(r=0.742,P<0.05).The expression level of serum miR-499a-5p was negatively correlated to Gensini score(r=-0.695,P<0.05),and TLR2 was positively correlated to Gensini score(r=0.557,P<0.05)in CHD patients.Compared with non-MACE group,the expression levels of serum miR-499a-5p decreased significantly,and TLR2 expression leveland Gensini score increased significantly in MACE group(P<0.05).The high level of miR-499a-5p was a protective factor(P<0.05),and high levels of TLR2 and Gensini score were risk factors for MACE occurrence(P<0.05).Conclusion The increase of TLR2 and decrease of miR-499a-5p level are closely correlated to MACE occurrence in CHD patients.