Effect of hyperoside on myocardial cell apoptosis and myocardial fibrosis in rats with chronic heart failure based on SIRT3/PPARγ pathway
Zhao Shucai
Ma Xuge
Lei Dazhou
Abstract：Objective To observe the effect of Hyperoside on myocardial cell apoptosis and myocardial fibrosis in chronic heart failure(CHF)rats and to explore the related mechanisms.Methods Thirty CHF rats were randomly divided into the CHF,hypericin,and combination groups,with 10 in each group.Another ten rats were sham-operated and set as the sham-operated group.The rats in the combination group were given hypericin(200 mg/kg)by intraperitoneal injection and intraperitoneally injected with 3-TYP[type Ⅲ histone deacetylase(SIRT3)/peroxidase proliferator-activated receptor gamma(PPARγ)signaling pathway inhibitor](50 mg/kg).In the Hyperoside group,hyperoside(200 mg/kg)was given intraperitoneally,and 3-TYP(50 mg/kg)was intraperitoneally injected with the same amount of DMSO.The sham-operated group and the CHF group were given the same volume of DMSO by gavage,and the same volume of DMSO was injected intraperitoneally one time a day for eight weeks of intervention.Doppler ultrasound was used to detect cardiac function in rats.Cardiomyocyte apoptosis was observed by Tunel staining.Masson staining was used to observe the degree of myocardial fibrosis.Western blot was used to detect the expression of SIRT3 and PPARγ protein in myocardial tissue.Results Compared with CHF group,left ventricular ejection fraction(EF),the maximum rate of left ventricular pressure rise(+dp/dtmax),the maximum rate of left ventricular pressure fall(-dp/dtmax),myocardial tissue SIRT3,and PPARγ protein expressions were increased,while the left ventricular end-diastolic pressure(LVEDP),myocardial cell apoptosis rate and collagen volume fraction(CVF)were decreased in the hyperoside group(P＜0.05).Compared with the hyperoside group,the EF,+dp/dtmax,-dp/dtmax,myocardial tissue SIRT3 and PPARγ protein expressions were decreased,while LVEDP,cardiomyocyte apoptosis rate,and CVF were increased in the combined group(P＜0.05).Conclusion Hyperoside can improve cardiac function in CHF rats and inhibit myocardial cell apoptosis and myocardial fibrosis,and its mechanism may be related to the activation of the SIRT3/PPARγ signaling pathway.
Keywords：HyperosideChronic heart failureCardiomyocytesApoptosisMyocardial fibrosis
Publication Date：2025-06-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 679-682,687 )