Study on the mechanism of LOX-1,eNOS and FFA signaling pathways in regulating microcirculation disorders of slow coronary blood flow
Yu Hui
Li Bin
Gan Shouyi
Abstract：Objective To investigate the role of plasma lectin-like oxidized low-density lipoprotein receptor-1(LOX-1),endothelial nitric oxide synthase(eNOS)and free fatty acids(FFA)in coronary slow blood flow(CSFP).Methods A total of 229 patients who underwent coronary angiography(CAG)examination in the Department of Cardiology of Xianning Central Hospital from August 2021 to July 2022 were taken as the study subjects.According to the results of CAG examination,the patients were divided into CSFP group(n=118)and control group(normal CAG examination,n=111).Plasma LOX-1 and eNOS were determined by double-antibody sandwich enzyme-linked immunosorbent assay(ELISA),and plasma FFA levels were determined by visible spectrophotometry.The number of thrombolysis test frames(TFC)of patients with myocardial infarction was recorded.Results Compared with the control group,the CSFP group had significantly increased plasma LOX-1,FFA,and mean(m)TFC(P＜0.05),and plasma eNOS decreased significantly(P＜0.001).Spearman correlation analysis showed that plasma LOX-1(r=0.577)and FFA(r=0.733)were significantly positively correlated with mTFC(P＜0.001),while eNOS(r=-0.342,P＜0.001)was negatively correlated with mTFC.The concentrations of plasma LOX-1,eNOS and FFA in predicting CSFP were 179.422 pg/ml,69.700 nmol/dl and 5.335 pg/ml,with specificity of 0.694,0.500 and 0.892,respectively,and the sensitivity of the three in predicting CSFP was＞0.7.Logistic multivariate regression model showed that plasma LOX-1,eNOS and FFA were independent predictors of CSFP(P＜0.05).Conclusion Plasma LOX-1 and FFA concentrations were increased in CSFP patients,while plasma eNOS levels were significantly reduced,and these three biomarkers could be used for the diagnosis of CSFP.
Keywords：Lectin-like oxidized low-density lipoprotein receptor-1Free fatty acidsEndothelial nitric oxide synthaseSlow coronary artery blood flow phenomenonThrombolysis in myocardial infarction frame count
Publication Date：2025-02-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 208-212 )