Effect of Guanxinning Injection in improving lymphangiogenesis to relieve myocardial ischemia injury
Zhang Yingxue
Zhu Sinan
Fang Zhirui
Xu Zhenkun
Bai Yuxin
Wang Hong
Chen Lu
Abstract：Objective To investigate the effective mechanism of Guanxinning Injection in improving lymphangiogenesis to relieve ventricular remodeling after myocardial infarction(MI)in mice.Methods Male C57BL/6 mice(n=100,aged 6～8 weeks old)were randomly divided into sham-operation group,MI model group,low-dose and high-dose Guanxinning Injection groups(low-dose group,high-dose group)and captopril group(each n=20).Except of sham-operation group,MI model was established through permanent ligation of left coronary anterior descending branch in other groups.The sham-operation group and MI model group were injected intraperitoneally with 0.1 ml of saline,the low-dose and high-dose groups were injected intraperitoneally with 2.5 ml/kg and 5 ml/kg of Guanxinning Injection,respectively,and captopril group was given orally 23.4 ml/kg of captopril once a day for 28 d.After treatment for 4 weeks,the levels of left ventricular ejection fraction(LVEF),left ventricular fraction shortening(LVFS),left ventricular end-diastolic diameter(LVEDD)and left ventricular end-systolic diameter(LVESD)were detected by using echocardiogram.The samples of cardiac tissue were collected for detecting cardiac pathological morphology after HE staining,and severity of myocardial fibrosis was detected after Masson staining.Expressions of leukocyte differentiation antigen 68(CD68)and lymphatic vascular endothelial receptor-1(LYVE-1)were detected by using immunofluorescence.The changes of LYVE-1 protein content were detected by using Western blotting assay,and CD68 proportion in mediastinal lymph nodes was detected by using flow cytometry.Results LVEF and LVFS decreased significantly in MI model group compared with sham-operation group(P＜0.05).Compared with IM model group,LVEF increased significantly,and LVEDD and LVESD decreased significantly(P＜0.05)in low-dose group,LVEF and LVFS increased significantly,and LVEDD decreased significantly(P＜0.05)in high-dose group.Compared with IM model group,MI area decreased in low-dose group,high-dose group and captopril group(P＜0.05).Compared with IM model group,the level of CD68 inflammatory infiltration in infarct border area decreased,protein and mRNA expressions of LYVE-1 increased(P＜0.05),and CD68 proportion in mediastinal lymph nodes increased(P＜0.05)in low-dose group and high-dose group.Conclusion Guanxinning Injection can significantly improve heart function,regulate inflammatory response in lymphatic restriction myocardial tissue in infarct border area,and provide theoretic basis for the effective mechanism of Guanxinning Injection in regulating cardiac lymphangiogenesis to promote the repair of myocardial ischemic injury.
Keywords：Myocardial infarctionGuanxinning InjectionLymphangiogenesisInflammatory responseMice
Publication Date：2025-02-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 175-179 )