Influence of Qihong San on myocardial energy metabolism in rats with heart failure:an investigation based on AMPK/PPARα signaling pathway
Fan Hui
Zhai Xueqin
Li Jiang
Wang Xiaofeng
Abstract：Objective To investigate the influence of Qihong San on myocardial energy metabolism and signaling pathway of AMP-activated protein kinase/peroxisome proliferator-activated receptor α(AMPK/PPARα)in rats with heart failure(HF).Methods Male SD rats(SPF grade,n=40,body weigh=200±220 g)were divided into sham-operation group,model group,Qihong San group and Qihong San+AMPK inhibitor group.HF was induced by using transverse aortic constriction(TAC)in rats,and after 28 d medication treatment,and left ventricular end-systolic diameter(LVESD),left ventricular end-diastolic diameter(LVEDD),left ventricular fraction shortening(LVFS)and left ventricular ejection fraction(LVEF)were detected by using cardiac ultrasonography.The pathological changes of rat myocardial tissue were detected by using H&E staining,and myocardial fibrosis was detected by using Masson staining.The levels of AMPK,phosphorylated AMPK(p-AMPK)and PPARα were detected by using Western blotting assay.The levels of superoxide dismutase(SOD),malondialdehyde(MDA),adenosine triphosphate(ATP)and reactive oxygen species(ROS)were detected by using enzyme-linked immunosorbent assay(ELISA).Results Compared with sham-operation group,LVEF and LVFS decreased,LVEDD and LVESD increased,levels of p-AMPK,PPARα,ATP and SOD decreased,disorderof myocardial fiber arrangement and inflammatory cell infiltrationincreased,and levels of MDA and ROS increased in model group(P<0.05).Compared with model group,LVEF and LVFS increased,LVEDD and LVESD decreased,levels of p-AMPK,PPARα,ATP and SOD increased,disorderof myocardial fiber arrangement and inflammatory cell infiltrationwere relieved,and levels of MDA and ROS decreased in Qihong San group(P<0.05).Compared with Qihong San group,the regulative effects of Qihong San on myocardial energy metabolism and oxidative damage were reversed in Qihong San+AMPK inhibitor group.Conclusion Qihong San can activite AMPK/PPARα signaling pathway,improve myocardial energy metabolism and relieve oxidative damage in HF rats.
Keywords：Myocardial energy metabolismQihong SanOxidative damageAMP-activated protein kinase/peroxisome proliferator-activated receptor αRats
Publication Date：2025-01-27
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 74-77,82 )