Study on the correlation between serum LncRNA-ZFAS1,miR-150-5p and cardiac function grading and prognosis in elderly patients with chronic heart failure

Wang Lili; Liu Yinghua; Xie Ming; Wang Yuxia; Liu Yaning; Zhang Xiaokun; Cui Shenqing

doi:10.3969/j.issn.1674-4055.2025.01.10

Abstract：Objective To investigate the correlation between serum LncRNA-ZFAS1,miR-150-5p,cardiac function grading,and prognosis in elderly patients with chronic heart failure(CHF).Methods From January 2021 to June 2022,100 elderly CHF patients who underwent treatment in General Hospital of North China Petroleum were collected,according to the New York Heart Association(NHYA)cardiac function classification,they were grouped into grade Ⅱ group(n=31),grade Ⅲ group(n=43),and grade Ⅳ group(n=26),they were separated into a good prognosis group(n=70)and a poor prognosis group(n=30)based on the prognosis,in addition,62 healthy volunteers who underwent physical examinations in our hospital were included as the reference group.QRT-PCR was applied to detect the relative expression levels of serum LncRNA-ZFAS1,miR-150-5p.Pearson method was applied to analyze the correlation between LncRNA-ZFAS1 and miR-150-5p,and their relationship with cardiac function indicators,receiver operating characteristic(ROC)curve was plotted to evaluate the predictive value of serum LncRNA-ZFAS1,miR-150-5p for the prognosis of elderly CHF patients.Results Compared with the reference group,the level of LncRNA-ZFAS1 in CHF patients was obviously increased,while the level of miR-150-5p was obviously decreased(P＜0.05);The difference between the levels of LncRNA-ZFAS,miR-150-5p in patients in the CHF class Ⅱ,class Ⅲ,and classⅣ groups was statistically significant(P＜0.05);the statistically difference between patients with or without heart disease,cardiac function indices,and LncRNA-ZFAS,miR-150-5p between patients in the poor prognosis group and the good prognosis group(P＜0.05);the serum LncRNA-ZFAS1 level in CHF patients was negatively correlated with LVEF and miR-150-5p,but positively correlated with LVEDD,LAD,and LVMI(P＜0.05),miR-150-5p was positively correlated with LVEF,negatively correlated with LVEDD,LAD,and LVMI(P＜0.05);the AUC of LncRNA-ZFAS1,miR-150-5p,and their combination was 0.831,0.851,and 0.910,respectively,the combined prediction of the two was obviously better than LncRNA-ZFAS1(Z=1.973,P=0.049),and miR-150-5p(Z=2.067,P=0.039)alone.Conclusion The level of LncRNA-ZFAS1 increases and the level of miR-150-5p decreases in elderly CHF patients and they correlate with cardiac function grading and prognosis.