Influence of Gualoupi Injection on cardiomyocyte injury and apoptosis induced by hypoxia/reoxygenation through regulating c-Jun N-terminal kinase/p38 mitogen-activated protein kinases pathway
Zhou Yutian
Ju Ling
Shi Lun
Hua Feng
Yin Jianfeng
Zhou Xiang
Zhou Kuo
Abstract：Objective To investigate the effect and possible metabolism of Gualoupi Injection in improving cardiomyocyte activity and inhibiting cardiomyocyte apoptosis in the condition of hypoxia/reoxygenation(H/R)injury through regulating pathway of c-Jun N-terminal kinase/p38 mitogen-activated protein kinases(JNK/p38).Methods The model of H/R was established by using H9c2 cardiomyocytes,and then divided into control group,H/R group,H/R+Gualoupi Injection group and H/R+Gualoupi Injection+anisomycin group.The cardiomyocyte activity was detected by using cell counting kit-8(CCK-8),and activities of lactic dehydrogenase(LDH)and Caspase 3 were detected by using kit assay in all groups.The cardiomyocyte apoptosis was detected by using TUNEL,and apoptosis-related proteins,including BCL-2,BAX,Pro-caspase 3 and Cleaved-caspase 3,were detected by using Western blotting assay(WB),and expressions of JNK/p38 pathway was detected in all groups.Results The cardiomyocyte activity decreased significantly,activities of LDH and Caspase 3 increased significantly,cardiomyocyte apoptosis rate increased,expressions of BAX,Pro-caspase 3 and Cleaved-caspase increased,Bcl-2 expression decreased,expressions of JNK/p38 pathway proteins(JNK,p-JNK,p38,p-p38)increased significantly in H/R group compared with control group(P＜0.05).The cardiomyocyte activity was raised in different groups treated by Gualoupi Injection compared with H/R group,especially in group treated with 2％of Gualoupi Injection(P＜0.05).The cardiomyocyte activity increased significantly,activities of LDH and Caspase 3 decreased,expressions of cardiomyocyte apoptosis rate and pro-apoptosis proteins(BAX,Pro-caspase 3,Cleaved-caspase 3)decreased,and expression of anti-apoptosis protein increased,and expressions of JNK/p38 pathway proteins(JNK,p-JNK,p38,p-p38)were inhibited in H/R+Gualoupi Injection group and H/R+Gualoupi Injection+anisomycin group compared with H/R group(P＜0.05).The cardiomyocyte activity was inhibited,activities of LDH and Caspase 3 increased,and expressions of BAX,Pro-caspase 3,Cleaved-caspase 3,NK,p-JNK,p38 and p-p38 increased significantly,and Bcl-2 expression decreased in H/R+Gualoupi Injection+anisomycin group compared with H/R+Gualoupi Injection group(P＜0.05).Conclusion Gualoupi Injection can improve cardiomyocyte activity and reduce apoptosis through inhibiting activation of JNK/p38 pathway.
Keywords：Pathway of c-Jun N-terminal kinase/p38 mitogen-activated protein kinasesGualoupi InjectionApoptosisHypoxia/reoxygenation injuryMyocardial protective effect
Publication Date：2024-10-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1231-1235 )