Effect of nicorandil targeting microRNA-22-3p for regulating pyroptosis on hyperglycemia-induced myocardial injury:a mechanism study
Chen Jun
Li Jianhao
Chen Guoqin
Abstract：Objective To discuss the mechanism of effect of nicorandil targeting microRNA-22-3p(miR-22-3p)for regulating pyroptosis on hyperglycemia-induced myocardial injury.Methods H9c2 cells,after routine culture,were divided into control group(untreated H9c2 cells),hyperglycemia-induced group(hyperglycemia-induced H9c2 cells),nicorandil group(hyperglycemia-induced H9c2 cells treated with nicorandil)and nicorandil+miR-22-3p mimics group(hyperglycemia-induced H9c2 cells treated with nicorandil+miR-22-3p).The expression of miR-22-3p in H9c2 cells were detected by using polymerase chain reaction(PCR),survival rate of H9c2 cells was detected by using methyl thiazolyl tetrazolium test(MTT),and apoptosis of H9c2 cells was detected by using AO/EB staining.The injury of H9c2 cells was observed with electron microscope.The pyroptosis-related proteins,including caspase-1,NOD-like receptor thermal protein domain associated protein 3(NLRP3)and apoptosis-related speck-like protein(ASC),were detected by using Western blotting assay.Results Compared with control group,miR-22-3p and cell survival rate decreased,and apoptosis rate,caspase-1,NLRP3 and ASC increased in hyperglycemia-induced group(P＜0.05).Compared with hyperglycemia-induced group,miR-22-3p and cell survival rate increased,apoptosis rate,caspase-1,NLRP3 and ASC decreased in nicorandil group(P＜0.05).Compared with nicorandil group,miR-22-3p and cell survival rate increased,and apoptosis rate,caspase-1,NLRP3 and ASC decreased in nicorandil+miR-22-3p mimics group(P＜0.05).The results of electron microscope observation showed that myocardial cell structure was intact in control group,and obvious cell swelling accompanied by severe contraction of cell membrane and nucleus were observed in hyperglycemia-induced group.The cell swelling and shrinkage were relieved in nicorandil group and nicorandil+miR-22-3p mimics group,which was more significant in nicorandil+miR-22-3p mimics group.Conclusion Nicorandil can relieve cardiomyocyte injury,pyroptosis status,cardiomyocyte apoptosis and myocardial injury to some extent through targeting miR-22-3p.
Keywords：Myocardial injuryNicorandilMiR-22-3pPyroptosis
Publication Date：2024-10-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1222-1226 )