Expression and significance of miR-125b-5p and miR-509-5p in serum of patients with acute myocardial infarction
Liu Fei
Zhao Qiang
He Chunlin
Yu Shuhua
Wang Fengyun
Abstract：Objective To investigate the expression and significance of serum microRNA-125b-5p(miR-125b-5p)and microRNA-509-5p(miR-509-5p)in patients with acute myocardial infarction(AMI).Methods A total of 126 AMI patients admitted to Department of Hypertension,Zhengzhou Central Hospital Affiliated to Zhengzhou University from April 2020 to April 2023 were regarded as the study subjects(AMI group),AMI patients were separated into Killip grade Ⅰ group(n=47),Killip grade Ⅱ group(n=44),and Killip grade Ⅲ group(n=35)based on Killip grading,another 126 volunteers who underwent physical examinations in our hospital were collected as the control group;the levels of serum miR-125b-5p,miR-509-5p,and myocardial injury related indicators[Creatine kinase isoenzyme(CK-MB),cardiac troponin Ⅰ(cTnⅠ),brain natriuretic peptide(BNP)]in each group were compared;Pearson method was applied to analyze the correlation between serum miR-125b-5p,miR-509-5p and myocardial injury related indicators in AMI patients;Logistic regression was applied to analyze the factors that affected the occurrence of AMI;receiver operating characteristic was applied to analyze the diagnostic value of serum miR-125b-5p and miR-509-5p for AMI.Results The serum levels of miR-509-5p,CK-MB,cTnⅠ,and BNP in the AMI group were obviously higher than those in the control group(P＜0.05),while the serum levels of miR-125b-5p and Left ventricular ejection fraction(LVEF)were obviously lower than those in the control group(P＜0.05);the serum miR-125b-5p level in AMI patients was negatively correlated with CK-MB,cTnⅠ,BNP levels,and positively correlated with LVEF(P＜0.05),the serum miR-509-5p level was positively correlated with CK-MB,cTnⅠ,BNP levels,and negatively correlated with LVEF(P＜0.05);as Killip grading increased,serum miR-125b-5p showed a gradually decreasing trend,while serum miR-509-5p showed a gradually increasing trend,the differences between the any two of the three groups were statistically obvious(P＜0.05);Logistic regression analysis showed that miR-509-5p,CK-MB,cTnⅠ,BNP were risk factors that affected the occurrence of AMI,while miR-125b-5p and LVEF were protective factors(P＜0.05);the sensitivity and specificity of the combination of serum miR-125b-5p and miR-509-5p in the diagnosis of AMI was 94.29％and 87.91％,respectively,the combined diagnostic efficacy was superior to their respective individual diagnoses(Zcombination-miR-125b-5p=2.531,Zcombination-miR-509-5p=2.243,P=0.011,P=0.025).Conclusion The serum miR-125b-5p level in AMI patients decreases,while miR-509-5p level increases,which is related to the levels of myocardial injury related indicators and the severity of the condition.The combination of the two has high diagnostic value for AMI.
Keywords：Micro RNA-125b-5pMicro RNA-509-5pAcute myocardial infarctionCreatine kinase isoenzyme
Publication Date：2024-08-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 960-964 )