Biomarkers and immune infiltration of dilated cardiomyopathy screened by weighted gene co-expres-sion network analysisand machine learning methodsand immune infiltration analysis
You Hongjun
Zhao Qianqian
Diao Jiayu
Gou Qiling
Shou Xiling
Dong Mengya
Abstract：Objective To screen biomarkers of dilated cardiomyopathy(DCM)by applying weighted gene co-expression network analysis(WGCNA)combined with machine learning methods,and analyze immune infiltration.Methods DCM transcription profilesfrom gene expression omnibus(GEO)was given a differential analysis.The differentially expressed genes(DEG)were analyzed based on gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis.WGCNA and LASSO regression were integrated to screen DCM biomarkers.The diagnostic efficacy of ROC curve was reviewed and immuneinfiltration was analyzed.Results There were 268 DEG in left ventricular tissue of DCM,which were mainly involved in regulation of inflammatory response,epithelial cell proliferation,collagen-containing extracellular matrix(ECM),ECM structural constituent,and meanwhile,took part in complement,coagulation cascades,phagosome,signaling pathway of JAK-STAT,arachidonic acid metabolism and signaling pathway of interleukin 17(IL-17).The identified biomarkers had higher diagnostic efficacy,including ASPN,CRYM,CSDC2,ECM2,FCN3,HMOX2,LAD1,SERPINA3,TSPYL2 and TUBA3D.The expressions of T helper 2 cell(Th2),central memory CD4+T cells and central memory CD8+T cells were significantly up-regulated in DCM myocardium.The expressions of activated dendritic cells,eosinophils,myeloid-derived suppressor cells,macrophages,mast cells,natural killer cells,neutrophils,regulatory T cells,follicular T helper cells,T helper 1 cells(Th1),T helper 17 cells(Th17)and effector memory CD8+T cells were significantly down-regulated.Conclusion ASPN,CRYM,CSDC2,ECM2,FCN3,HMOX2,LAD1,SERPINA3,TSPYL2 and TUBA3D may be taken as biomarkers for DCM.A variety of immune cells may mediate the occurrence and development of DCM.
Keywords：Dilated cardiomyopathyMachine learningBiomarkersImmune infiltration
Publication Date：2024-05-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 517-522 )