Establishment of diabetic cardiomyopathy rat model and evaluation index
Han Jiarong
Zhang Wenxiang
Sun Longfei
Chen Pengde
Yao Lan
Abstract：Objective To construct a rat model of diabetic cardiomyopathy and establish its evaluation index.Methods 30 SPF Wistar male rats were randomly divided into two groups according to body weight.The normal group was fed a conventional diet,and the model group was fed a high-sugar and high-fat diet for four weeks.Rats in the model group fasted for 12 hours and were intraperitoneally injected with streptozotocin(STZ)25 mg/kg.Blood samples at the tail tip were taken to detect random blood glucose after≥16.7 mmol/L and determined as type 2 diabetes rats.During the experiment,rats'body weight and blood glucose values were recorded once a week.After eight weeks,the heart index was calculated,and four indexes of blood lipid,electrocardiogram,and landmark myocardial enzymes were detected in each group.Masson and HE staining were used to observe the degree of fibrosis and morphological changes of rat myocardial tissue,and the volume fraction of myocardial Collagen was calculated.Western Blot detected the protein expression levels of Bax,Bcl-2,Caspase-3,TGF-β1,α-SMA,Collagen-ⅰ,TNF-α,and IL-6 in rat heart tissue.Results Compared with the normal group,the blood glucose value of the model group was significantly increased from week 1 to week 8(P<0.05),body weight was significantly decreased from week 3(P<0.05),Heart index was significantly increased(P<0.05),and Heart electrophysiological indexes were expressed as Heart rate(Heart rate,HR),QRS interval,ST segment amplitude,and QT interval level were significantly increased(P<0.05).The contents of triglyceride(TG),cholesterol(TC),low-density lipoprotein(LDL-C),lactate dehydrogenase(LDH),creatine kinase isoenzyme(CK-MB),and malondialdehyde(MDA)were significantly increased(P<0.05).The contents of high-density lipoprotein(HDL-C)and superoxide dismutase(SOD)were significantly decreased(P<0.05).HE staining showed that myocardial cells in the model group were disordered,and the space was enlarged,accompanied by inflammatory cell infiltration.Masson staining showed that compared with the normal group,myocardial collagen volume fraction in the model group was significantly increased(P<0.05),and fibrosis was severe.The protein expression levels of Bax,Caspase-3,TGF-β1,α-SMA,Collagen-ⅰ,TNF-α,and IL-6 in the myocardial tissue of the model group were significantly increased(P<0.05).In contrast,the protein level of Bcl-2 was significantly decreased(P<0.05).Conclusions High sugar and high-fat feed for four weeks intraperitoneal injection of low-dose STZ 25 mg/kg,continued to 8 weeks after feeding Wistar rats to a certain extent,has appeared similar to the clinical manifestations of diabetic cardiomyopathy.This method can be used to construct animal models of diabetic cardiomyopathy.These measurements can be used for diabetic cardiomyopathy in the rat evaluation model.
Keywords：DiabeticCardiomyopathyAnimal modelThe evaluation index
Publication Date：2024-03-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 328-334 )