Diagnostic value of circulating miRNA-26a-5p,miRNA-21-5p and miRNA-191-5p in elderly patients with acute myocardial infarction
Yang Xinying
Cao Xuanchao
Xu Xianjing
Wang Xinhui
Li Deyong
Huang Gairong
Abstract：Objective The purpose of this study was to compare the expressions of miRNAs in AMI patients and normal healthy people,and to review the diagnostic value of circulating miR-26a-5p,miR-21-5p and miR-191-5p to AMI.Methods Elderly AMI patients(n=60,AMI group)and healthy controls(n=80,control group)were chosen continuously from Department of Geriatrics in Henan Provincial People's Hospital from May 2020 to Dec.2020.The expression levels of circulating miR-26a-5p,miR-21-5p and miR-191-5p were detected by using qRT-PCR in 2 groups.The diagnostic value of serum miRNAs in AMI patients was analyzed by using ROC curve.Results The expression levels of miRNAs were by 1.8 to 2.0 times fold lower in AMI group compared with control group 2-ΔCt expression level:miR-26a-5p[(0.62±0.34)vs.(1.24±0.63),P<0.001],miR-21-5p[(0.87±0.56)vs.(1.56±0.91),P<0.001]、miR-191-5p[(0.99±0.51)vs.(1.88±1.04),P<0.001].The diagnostic value of MiR-26a-5p(AUC:0.806,95%CI:0.727～0.886,P<0.001)was higher than that of miR-21-5p(AUC:0.740,95%CI:0.651～0.828,P<0.001)and miR-191-5p(AUC:0.772,95%CI:0.689～0.855,P<0.001).AUC of combined 3 miRNAs was 0.864(95%CI:0.929～0.799,P<0.001),sensitivity was 93.33%(95%CI:83.8%～98.2%)and specificity was 65.00%(95%CI:51.6%～76.9%).Conclusion MiR-26a-5p,miR-21-5p and miR-191-5p are effective biomarkers for diagnosing myocardial infarction,which can provide a new idea to AMI diagnosis.
Keywords：Senile acute myocardial infarctionBiomarkersCirculating miRNAs
Publication Date：2024-03-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 288-291,295 )