To explore the therapeutic mechanism of Salvia miltiorrhiza and Rhodiola rosea on myocardial ischemia based on network pharmacology and molecular docking technology
Ji Zhao
Meng Xinyuan
Li Yu
Wang Huimin
Yue Yuanjia
Rong Xing
Jiang Lin
Abstract：Objective To investigate the potential mechanism of salvia miltiorrhist-Rhodiola in the treatment of myocardial ischemia(MI)based on network pharmacology and molecular docking techniques.Methods The active components of salvia miltiorrhiza and Rhodiola rosea were screened based on TCMSP and literature.The target of MI could be determined from GeneCards and other databases.After that,the intersection location between the corresponding target of the active ingredient and the disease target is selected.In addition,the STRING database was introduced to analyze the influence of each target on each other,and the Cluster Profiler package in R software was used to analyze biological functions and signal path enrichment.Based on the Cytoscape application,the protein-protein correlation and component target link system are established to determine the main active substances and targets.Vina software is used to interface core target proteins with drug-active ingredients.Results A total of 342 potential drug targets,2241 potential MI targets and 201 common targets were obtained.GO enrichment analysis yielded 3276(P<0.05)samples.By KEGG enrichment analysis,188 samples were obtained(P<0.05).The results of molecular docking showed that the target protein was closely bound to the active ingredient.Conclusion Salviorrhiza-Rhodiola pair may act on STAT3,JUN,AKT1 and MAPK3 to regulate inflammation,apoptosis and proliferation as the molecular mechanism of MI treatment,which reflects the characteristics of Chinese medicine treating MI through multi-target and multi-pathway,and provides new ideas for further revealing its pharmacological basis.
Keywords：Network pharmacologyMolecular dockingSalvia miltiorrhiza-Rhodiola roseaMyocardial ischemia
Publication Date：2024-02-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 133-139,143 )