Protective effect of stromal interaction molecule-1 inhibitor SKF96365 on atherosclerosis
Zhang Jing
Zhou Yuquan
Wan Xueqi
Liu Libo
Song Xiantao
Ge Changjiang
Abstract：Objective To investigate the protective effect of stromal interaction molecular 1(STIM1)inhibitor SKF96365 in atherosclerosis and its underlying mechanism.Methods Ten C57BL/6J mice were used as normal controls and 30 ApoE-/-mice were given a high-fat diet to establish an atherosclerosis(AS)model,of which 10 ApoE-/-mice were left untreated(model group);10 ApoE-/-mice were treated with atorvastatin calcium(10 mg/kg·d)continuously by gavage for 8 weeks from 16 weeks of age(statin treatment group);10 ApoE-/-mice were treated with SFK96365(10 mg/kg·d)continuously by subcutaneous injection for 8 weeks from 16 weeks of age(SKF96365 treatment group).The lipid profiles as well as pathological changes atherosclerotic plaques were observed;the expression of STIM1,pJNK,NF-κB and MMP-9 proteins were detected by western blot.Results Total cholesterol(TC),triglycerides(TG),and low-density lipoprotein cholesterol(LDL-C)were significantly lower in the SKF96365 group than in the model group(P＜0.05).p-JNK,NF-κB,and MMP-9 proteins were significantly lower in the SKF96365 group than in the model group(P＜0.05).Conclusion SKF96365 may increase plaque stability by attenuating oxidative stress and inflammatory damage in the arteries of ApoE-/-mice and by affecting the expression of MMP-9 protein activity.
Keywords：AtherosclerosisSKF96365InflammationOxidative Stress
Publication Date：2023-10-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1212-1215,1228 )