Effect of SGLT 2 inhibitor on the expression of inflammatory factors in diabetic mice
Zhang Zhigang
Sun Duirong
Huang Yangxia
Guo Lisha
Ren Lina
Sun Yufei
Yan Chao
Lei Xinyu
Abstract：Objective To investigate the effect of SGLT2 inhibitor on vascular endothelial inflammatory factors in diabetic mice.Methods 8-week-old CIR mice were injected intraperitoneally with low-dose STZ(50 mg/kg)to establish a diabetic model,and the modeling lasted for six days.The control groups were injected subcutaneously with normal saline,the model group(common feed),and the treatment group(common feed&dapagliflozin intervention treatment.Body weight and blood glucose changes were observed at the end of 4w,8w,and 12w.The expression of TNF-a,IL-1β,and MCP-1 in plasma was detected by the EILSA method,and the expression of inflammatory factors in the vascular wall was detected by Western blot.Results There were significant differences in body weight and blood glucose(P＜0.05).Compared with the control group,the expression of TNF-a in the 4W model group increased significantly(P＜0.01).However,the expression of IL-1β and MCP-1 in the 4W model group increased,but there was no significant difference(P＞0.05).TNF-a,IL-1β,and MCP-1 levels in plasma were significantly increased in the model groups of 8w and 12w(P＜0.05).Compared with the model group,the expression of TNF-a,IL-1β,and MCP-1 in the plasma of the treatment group did not change significantly at 4w(P＞0.05).At the 8w and 12w,the expression of TNF-a,IL-1β,and MCP-1 in the plasma of the model group decreased significantly(P＜0.05).The expression of inflammatory factor protein in vascular tissue was changed in the 12w(P＜0.05).Conclusions SGLT2 inhibitors effectively inhibited the release of inflammatory factors from the endothelium of diabetic mice.
Keywords：SGLT2 inhibitorsDiabetesInflammatory factors
Publication Date：2023-09-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1063-1066 )