Influence of ticagrelor on arrhythmia and calcium channel in rats after myocardial infarction reperfusion injury

Cai Qingqing; Ding Chao; Gao Yasong; Nie Weizhuo; Su Yanyan

doi:10.3969/j.issn.1674-4055.2019.11.27

Abstract：study the influence of ticagrelor on arrhythmia and L-type calcium channel in ventricular myocytes in animal model of myocardial infarction reperfusion injury. Methods Male and healthy SD rats (n=24) were divided randomly into 3 groups (each n=8): sham-operation group (without ligation), ischemia reperfusion model group (I/R group, ischemia for 30 min and reperfusion for 120 min) and ticagrelor group (20 mg/kg of ticagrelor orally 2 h before ligation). Lead-II of electrocardiograph was monitored, and the incidence of arrhythmias including ventricular premature beat (VPB), ventricular tachycardia and ventricular fibrillation (VF) were observed in 3 groups after reperfusion. The changes of L-type calcium channel current in ventricular myocytes were recorded by using whole cell patch clamp in all groups. Results Compared with I/R group, the number of VPB decreased significantly, and incidence rate of ventricular tachycardia and VF decreased significantly in ticagrelor group (P<0.05). The comparison in peak current densities of L-type calcium channel in 3 groups showed that it increased significantly in I/R group than that in sham-operation group [(-9.4±0.6) pA/pF vs. (-4.3±0.5) pA/pF, P<0.05], and decreased significantly in ticagrelor group than that in I/R group [(-7.1±1.0) pA/pF vs. (-9.4±0.6) pA/pF, P<0.05]. Compared with I/R group, the inactivation process of L-type calcium channel was significantly accelerated (P<0.05) and activation process was not affected (P>0.05) in ticagrelor group. Conclusion Ticagrelor can reduce the incidence rate of arrhythmia through decreasing significantly L-type calcium channel current in animal model with myocardial ischemia after reperfusion injury.