Influence of procyanidine B2 on oxidative damage of human umbilical vein endothelial cell line induced by hydrogen peroxide
Shen Jianzhong
Yao Yuan
Liu Dawei
Abstract：study the influence of procyanidine B2 (PB2) on oxidative damage of human umbilical vein endothelial cell line (EA.hy926) induced by hydrogen peroxide (H2O2) and possible mechanism. Methods EA.hy926 were cultured in vitro, and divided randomLy into 5 groups: control group, H2O2 group, α-tocopherol group (30 μM), PB2 group 1 (2 μM) and PB2 group 2 (10 μM). The control group was treated with DMEM, H2O2 group was treated with DMEM and H2O2 (final concentration=200 μmol/L), α-tocopherol group was treated with DMEM, H2O2 (final concentration=200 μmol/L) and α-tocopherol ((final concentration=30 μmol/L), PB2 group 1 was treated with DMEM, H2O2 (final concentration=200 μmol/L) and PB2 (final concentration=2 μmol/L), and PB2 group 2 was treated with DMEM, H2O2 (final concentration=200 μmol/L) and PB2 (final concentration=10 μmol/L). The growth of EA.hy926 was analyzed, and influence of PB2 on DNA injury, lipid peroxiation and reactive oxygen species (ROS) of EA.hy926 induced by H2O2 was detected. Results Compared with control group, the number of EA.hy926 decreased significantly in H2O2 group (P<0.05). Compared with H2O2 group, the number of EA.hy926 increased significantly in α-tocopherol group, PB2 group 1 and PB2 group 2 (all P<0.05). Compared with control group, DNA injury of EA.hy926 was more significant in H2O2 group (P<0.05). Compared with H2O2 group, DNA injury of EA.hy926 was significantly inhibited in PB2 group 1, PB2 group 2 and α-tocopherol group (P<0.05), and inhibition rate was, respectively, 24%, 55% and 95%. Compared with control group, the levels of malondialdehyde (MDA) and ROS increased significantly in H2O2 group (P<0.05). Compared with H2O2 group, the productions of MDA and ROS in EA.hy926 induced by H2O2 were significantly inhibited in PB2 group 1, PB2 group 2 and α-tocopherol group (P<0.05), and the inhibition rate was, respectively, 21%, 42% and 49%, and 18%, 41% and 80%. Conclusion PB2 can significantly inhibit the production of ROS in EA.hy926 induced by H2O2, relieve oxidative damage and protect endothelial cells.
Keywords：Procyanidine B2Hydrogen peroxideOxidative damage of human umbilical vein endothelial cellsReactive oxygen species
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1358-1361,1365 )