Influence of Survivin on apoptosis of vascular endothelial cells induced by Kringle 5 through mitochondrial pathway
Hu Qingfeng
Zhao Honglei
Bai Tao
Xue Jinrong
Liu Yongmin
Sun Lizhong
Abstract：discuss the effect of Survivin on apoptosis of vascular endothelial cells induced by Kringle 5 (K5). Methods The human umbilical vascular endothelial cells (HUVEC) were isolated and cultured and then treated with K5. The expression of Survivin was detected by using qRT-PCR and Western blotting assay. The overexpression vectors of Survivin were transfected in HUVEC and meanwhile HUVEC were treated with K5. The apoptosis was detected by using flow cytometry. The mitochondrial membrane potential (MMP) was detected by using JC-1 method, synthesis level of adenosine triphosphate (ATP) and activities of Caspase-3 and Caspase-9 were detected by using chromatoptometry. The level of reactive oxygen species (ROS) was detected by using H2DCFDA method. The level of cytochrome C (Cyt C) in mitochondrion and cytoplasma was detected by using Western blotting assay. Results After K5 treatment, the transfection and expression of Survivin decreased, and increased after transfected overexpression vectors of Survivin in HUVEC. After K5 treatment in HUVEC with overexpression, apoptosis decreased, MMP increased, ATP synthesis increased, activities of Caspase-3 and Caspase-9 decreased, ROS level decreased, and Cyt C level increased in mitochondrion and decreased in cytoplasma (P<0.05). Conclusion K5 can induce the down-regulation of Survivin expression, and up-regulation of Survivin can inhibit HUVEC apoptosis induced by K5.
Keywords：SurvivinMitochondrial pathwayVascular endothelial cellsKringle 5
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1311-1314 )