Expressions of insulin-like growth factor-1 and insulin-like growth factor-1 receptor in infracted myocardial tissue and intervention on them in rats
Yu Junmin
Zhang Xiaobo
Li Dahe
Wang Huidong
Zhao Yuanyuan
Shen Weiwei
Abstract：investigate the effect of pathways of insulin-like growth factor-1 (IGF-1) and insulin-like growth factor-1 receptor (IGF-1R) in myocardial infarction and influence of irbesartan on them through observing the changes of myocardial IGF-1 mRNA and protein and IGF-1R mRNA and protein. Methods The anterior descending branch of left coronary artery was ligatured in rats, and after 24 h the survived rats were randomly divided into medicinal group and control group (each n=9). A sham-operation group was established (n=9) and did not given ligation. The medicinal group was treated with irbesartan (50 mg·kg/d) and control group and sham-operation group were orally given normal saline (NS) in the same volume once a day. After 4 weeks all rats were sacrificed. The myocardial pathological changes were observed after HE staining, and levels of IGF-1 mRNA and protein and IGF-1R mRNA and protein were detected by using real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) and Western blotting assay. Results The results of HE staining showed aligned cardiomyocytes in sham-operation group, and swelling cardiomyocytes and infiltration of inflammatory cells in medicinal group and control group. These changes were more significantly in control group than those in medicinal group. The weight of left ventricle was lower in medicinal group than that in control group (P<0.05). The IGF-1 mRNA and protein and IGF-1R mRNA and protein were detected in all groups, and they were higher in medicinal group and control group than those in sham-operation group (all P<0.05). The levels of IGF-1 mRNA and protein and IGF-1R mRNA and protein were lower in medicinal group than those in control group (all P<0.05). Conclusion The expressions of myocardial IGF-1 mRNA and protein and IGF-1R mRNA and protein can be detected in rat myocardial tissue. The levels of IGF-1 mRNA and protein and IGF-1R mRNA and protein will increase after myocardial infarction. Irbesartan can significantly reduced the levels of myocardial IGF-1 mRNA and protein and IGF-1R mRNA and protein in myocardial tissue and relieve myocardial damage in rats with myocardial infarction after applied for 4 weeks.
Keywords：Insulin-like growth factor-1Insulin-like growth factor-1 receptorMyocardial infarctionIrbesartanRats
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1201-1204 )